The Tumour Microenvironment

The brief also had a second challenge: much of the audience wouldn't be fluent in transcriptional biology.  Fusion built a 3D medical animation that explained the underlying transcription biology clearly and quickly enough to enable viewers to focus on the nuances of the mechanism of action (MoA).
We built the film as a single continuous journey from the lung, through the microenvironment, into the cancer cells and down into the nucleus to help orient the viewer. We then focussed on the specific guanine-containing triplets on the DNA strand where the lurbinectedin binds.
A split screen enabled us to compare the downstream impact with and without the drug. This helped us to show the lack of something (a common challenge we face). In this case, a lack of transcription, which in turn stops protein production.
Once the principles of the impact of inhibiting transcription had been shown, we could then move on to the TAMs within the tumour microenvironment. Here we were able to pull back and show the impact of lurbinectedin on cytokine production without needing to go back into the nucleus of the TAMs